Liftoff.bio illustration — cat genomics

Salmon

Fast, bias-aware transcript quantification from RNA-seq

Salmon quantifies transcript abundance from RNA-seq reads using lightweight selective alignment against a transcriptome, correcting for GC and sequence-specific biases.

Example

1salmon index -t transcripts.fa -i salmon_index
2salmon quant -i salmon_index -l A -1 r1.fq -2 r2.fq -p 8 -o quant

Outputs integrate cleanly with tximport and downstream differential-expression tools.

Related tools

Liftoff.bio illustration — cat genomics
Open source

Genomics & Sequencing

SAMtools

The standard toolkit for SAM/BAM/CRAM alignment files

Command line
Liftoff.bio illustration — cat single cell
Open source

Single-Cell & Spatial

Scanpy

Scalable single-cell analysis in Python

Python library