Liftoff.bio
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Open sourceAI ModelsStructural Biology

BindCraft

One-shot de novo binder design with AlphaFold2 backpropagation

Source codePublication

BindCraft is a user-friendly, automated binder-design pipeline that backpropagates through AlphaFold2 to hallucinate binders with nanomolar affinity — often without a known binding site and without high-throughput screening.

Highlights

  • Reported experimental success rates of 10–100% across challenging targets.
  • Combines AF2 backpropagation, ProteinMPNN sequence design and PyRosetta scoring/filtering.
  • Validated against cell-surface receptors, allergens, de novo proteins and CRISPR–Cas9.

Note: PyRosetta components are free for non-commercial use only.

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