CHOPCHOP
Design and evaluate CRISPR guide RNAs
CHOPCHOP is a widely used tool for designing CRISPR guide RNAs for knockout, knock-in, activation and repression. It combines on-target efficiency scoring with genome-wide off-target searching.
Highlights
- Supports Cas9, Cas12a (Cpf1), nickases and base editing.
- Visualizes guides on gene models with efficiency/specificity scores.
- Web interface plus a command-line version for batch design.
